Effects of Sophorae Radix on Human Gastric and Colorectal Adenocarcinoma Cells -Sophorae Radix and Cancer Cells-

摘要

Objectives: The purpose of this study was to compare the antioxidant and anti-inflammatory effects of Alum (AL) and BurntAlum (BAL), which are commonly used as external ointments.Methods: Extracts of AL and BAL were classified into three groups: 20, 50, and 100 /. The cytotoxicity was measured byusing MTT assays in human keratinocyte cell line (HaCaT). The anti-oxidant effect was measured by using the DPPH (1, 1-diphenyl-2-picryl-hydrazyl-hydrate) radical scavenger. The anti-inflammatory effect was measured by using the inhibitoryefficacy for the amount of nitric-oxide (NO) produced in mouse macrophage cell line (RAW 264.7).Results: BAL showed a higher level of cytotoxicity than AL. The AL groups showed a concentration-dependent scavengingeffect on DPPH radicals, but no significant relevance was found. The BAL groups showed a concentration-dependentscavenging effect on DPPH radicals. The scavenging effects of the BAL groups were almost insignificant, but the values forthe 20, 50, and 100 / trials were different. The BAL groups showed significant concentration-dependent inhibitory effects onNO production, but the AL groups did not.Conclusions: AL showed an anti-oxidant effect more efficiently than BAL did, which demonstrated a superior anti-inflammatory effect. Therefore, for external usage, AL must be distinguished from BAL.