Effect of methyl jasmonate on isoflavonoid accumulation and antioxidant enzymes in Pueraria mirifica cell suspension culture

摘要

The effect of methyl jasmonate (MJ) on the isoflavonoid accumulation and activities of three antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were studied in Pueraria mirifica cell suspension culture after MJ elicitation for up to 6 days. Treatment with MJ at a concentration of 1.0 µg/ml induced the highest isoflavonoid contents at the first day after elicitation, when compared to the control. This optimum concentration of MJ induced an oxidative stress in P. mirifica cells. The activity levels of superoxide dismutase and glutathione peroxidase were both increased, whilst that for catalase was decreased in the MJ-treated cells. Thus, P. mirifica cells have a defense mechanism against superoxide and hydrogen peroxide stress and MJ is one such potential stimulator of this defense mechanism.