PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY,CULTIVABILITY AND METABOLIC CHANGES OF E. COLI USINGDIFERENT MESUREMENTS METHODS

摘要

In this work, the oxidative stress was produced by the illumination of either fixed TiO2 or suspended TiO2, andthe relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) andFluorescence In Situ Hybridisation (FISH) methods. Under certain oxidative conditions simulating incomplete treatments,viable cells that had lost their cultivability were observed to recover all of their physiological functions when transferred tofresh nutrient media. Moreover, alterations in the nutrient assimilation processes were studied by 1H-NMR during and afterthe photocatalytic treatment. This allowed us to shed some light on how the damage chain propagates during the treatment.This work demonstrate that the Standard Plate Count (SPC) method commonly used to estimate the c ell populations duringdisinfection processes is ill adapted, whereas the FISH-DVC pair offers a reliable alternative.