Optimization of media components for the production of laccase from Pleurotus ostreatus PKN 04 using Response surface methodology

摘要

The influence of media components plays an important role on the enhanced production of products from microorganism. An approach was done to enhance the production of laccase from Pleurotus ostreatus – white rot fungi by manipulating the different concertation of variables using Response surface methodology. The Enzyme production was correlated with the biomass produced during the production process.6 g/L NaNO3, 1.5 g/L K2HPO4, 1 g/L Tryptone, 10 g/L Glucose, 0.5 g/L MgSO4 combination showed maximum enzyme activity of 96 IU/ml with an agreeable increase in biomass (5.2 g/L). 50 experiments runs were attained this optimum conditions for enzyme production and the coefficient of determination was 99.5%.