Isolation, Screening, Characterization of PGPR of Lentils Lens culinaris

摘要

In plant growth-promoting rhizobacteria (PGPR) are soil bacteria that colonize plant roots and improve their growth by different ways. The main aim of the present study was the isolation and the characterization of the rhizospheric bacterial strains belonging to the genus of Bacillus, which were responsible for the growth of Lens culinaris. For this purpose, five samples of soils were collected from several region of Sidi Bel Abbès located, located in North West of Algeria such as Ain Témouchent, Tessala, ITCMI, Sfisef and Kaid Belarbi, which were investigated for their capability of promoting plant growth of Lens culinaris. Twenty four bacterial strains belonging the of the genus of Bacillus were isolated and initially selected by the study of germination process in vitro by the determination of some parameters such as root length, stem length, dry weight and fresh grain weight like the B8, B11, B14 and B15. Furthermore, the isolated, selected promoting growth bacterial strains were subsequently characterized by the study of indol-2-acetic acid (IAA) production, phosphate solubilization, nitrogen assimilation and screened for their antifungal activity against Fusarium oxysporum. The obtained results indicated that the isolated, selected bacterial strains (B15, B10) has manifested a excellent antagonistic activity against Fusarium oxysporum, with a maximal diameter of inhibition zone 50, 50 mm respectively. Whereas, the both isolates (B5, B6) have showed a average, with a maximal diameter of inhibition zone 25, 25 mm respectively. Moreover, the isolated, selected bacterial strains (B4, B7, B12) has indicated the lowest antagonistic activity against Fusarium oxysporum, with a diameter of inhibition zone of 10 mm. Furthermore, the both selected bacterial (B4, B15) were further characterized by the phosphorus solubilization with the varied Furthermore, the results revealed that the isolated strains presented similar characteristics to the genus of Bacillus sp, where both isolated, selected bacterial strains B7 and B15 has presented an excellent effect for promotion germination of Lens culinaris