Plasmid profiling with respect to identification of multidrug resistance inStaphylococcus aureus isolated from dairy products

摘要

Present study to examine the Plasmid profiling with respect to identification of multidrug resistance in Staphylococcus aureus separated from dairy products. In this study S. aureus were separated from milk and its products on selective Mannitol Salt Agar (MSA) medium which was followed by gram staining and biochemical characterization. The gram staining results demonstrated that graphs like cluster of cell of S. aureus whereas biochemical test results also showed that positive for S. aureus. Antibiotic resistance was identified by disc diffusion method. The antibiotic resistant pattern of S. aureus isolate was tested against 8 commercially available antibiotics as Ampicillin, Gentamicin, Kanamycin, Ciprofloxacin, Cefotaxime, Bacitracin, Methicillin and Penicillin. Results showed that isolated organism showed resistance to Methicillin, Bacitracin and Penicillin. Intermediate level of resistance was recorded in Ciprofloxacin and Cefotaxime. Isolated S. aureus were sensitive for the antibiotics such as Ampicillin, Gentamicin and Kanamycin. The percentage occurrence of Multiple Antibiotic Resistance (MAR) index was the highest (70%) for the MAR index of 0.8. The plasmids were separated and bands (1500-100bp) were observed followed by electrophoresis. The antibiotic resistant genes was identified by PCR. The result showed that 7 isolated strains which carried on mec gene (1000bp-100bp). This mec gene was resistant to Methicillin, Penicillin and Bacitracin. Conclusion: Ampicillin, Gentamicin and Kanamycin were active against the isolated organisms which can be used for treatment of bovine mastitis in cattle.