Antimicrobial activity and cytotoxicity of Theobroma cacao extracts

摘要

The cocoa leaf (CL), seeds (CS) and pod (CP) were studied for the estimation of medicinal properties that they contain and the focus was screening for anticancer ability. Extraction of the samples in Soxhlet apparatus was done with methanol as the solvent followed by phytochemical analysis, antimicrobial activity, antioxidant assay and anticancer activity. Thin Layer Chromatography (TLC) showed the number of compounds in hexane:ethyl acetate: methanol solvent system for cocoa pod and chloroform:methanol solvent for cocoa leaf under UV illuminator. The antimicrobial assay by agar well diffusion method of cocoa seeds showed zone of inhibition against pathogens Serratia marcescens, Staphylococcus aureus, Salmonella sp. and Shigella dysenteriae. Further, antioxidant assay was performed using DPPH radical scavenging assay. The sample was then tested for cytotoxicity assay against MG63 osteosarcoma cell lines and active compounds were identified by doing Gas chromatography and Mass spectroscopy (GCMS).