Development and validation of kinetic spectrophotometric method for the determination of lamivudine in pure and commercial tablets

摘要

A rapid, simple and sensitive spectrophotometric method for the determination of lamivudine has been developed and validated. The method is based on oxidation of lamivudine with alkaline potassium permanganate. The reaction is kinetically studied by measuring the rate of change of absorbance at 610nm. The rate constant, fixed absorbance and fixed time methods are investigated. Only the fixed time method is utilized for construction of calibration graph to determine the concentration of the studied drug. The results are validated statistically and checked through recovery studies. The method has been successfully applied for the determination of lamivudine in its commercial dosage form.