Evaluation of the Methanol Extract of Yazd Native Plants on Peripheral Blood Mononuclear Cell Proliferation and IL-4 Secretion

摘要

Introduction: Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis are defined as native plants of Yazd that their immunomedulatory effects have not been studied yet. The aim of this study was to determine the effect of different concentrations of these plants on peripheral blood mononuclear cells(PBMCs) proliferation and interleukin(IL)-4 secretions. methods: Root extracts of Echinops ilicifolius, Echinops jesdianus, Echinops ceratophorus and Echinops lasiolepis were prepared by Maceration method. PBMCs were obtained from three healthy volunteer individuals and cultured with the presence of 0.1,1,10,100 and 200 μg/ml with concentrations of 10 μg/ml of phytohemagglutinin. The rate of cell proliferation was determined by BrdU kit. The IL-4 levels in PBMCs. supernatant were measured by enzyme-linked immunosorbent assay(ELISA). P value