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Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

机译:梅森-辉瑞猴病毒包膜糖蛋白的循环及其与未成熟颗粒的水泡共转运。

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摘要

The envelope glycoprotein (Env) plays a crucial role in the retroviral life cycle by mediating primary interactions with the host cell. As described previously and expanded on in this paper, Env mediates the trafficking of immature Mason-Pfizer monkey virus (M-PMV) particles to the plasma membrane (PM). Using a panel of labeled RabGTPases as endosomal markers, we identified Env mostly in Rab7a- and Rab9a-positive endosomes. Based on an analysis of the transport of recombinant fluorescently labeled M-PMV Gag and Env proteins, we propose a putative mechanism of the intracellular trafficking of M-PMV Env and immature particles. According to this model, a portion of Env is targeted from the trans-Golgi network (TGN) to Rab7a-positive endosomes. It is then transported to Rab9a-positive endosomes and back to the TGN. It is at the Rab9a vesicles where the immature particles may anchor to the membranes of the Env-containing vesicles, preventing Env recycling to the TGN. These Gag-associated vesicles are then transported to the plasma membrane.
机译:包膜糖蛋白(Env)通过介导与宿主细胞的主要相互作用,在逆转录病毒的生命周期中起着至关重要的作用。如前所述并在本文中进行扩展,Env介导了未成熟的Mason-Pfizer猴病毒(M-PMV)颗粒向质膜(PM)的运输。使用一组标记的RabGTPases作为内体标记,我们鉴定到Env主要存在于Rab7a和Rab9a阳性内体中。基于对重组荧光标记的M-PMV Gag和Env蛋白运输的分析,我们提出了M-PMV Env和未成熟颗粒在细胞内运输的推测机制。根据该模型,Env的一部分从反高尔基网络(TGN)靶向Rab7a阳性内体。然后将其转运至Rab9a阳性内体,然后转运回TGN。在Rab9a囊泡中,未成熟的颗粒可能锚定在含Env囊泡的膜上,从而阻止了Env循环到TGN。然后将这些与Gag相关的囊泡转运至质膜。

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