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A method for diagnosing vesicular stomatitis using envelope glycoprotein antigen of vesicular stomatitis virus and monoclonal antibody against the same
A method for diagnosing vesicular stomatitis using envelope glycoprotein antigen of vesicular stomatitis virus and monoclonal antibody against the same
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机译:利用水泡性口炎病毒的包膜糖蛋白抗原和针对其的单克隆抗体诊断水泡性口炎的方法
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摘要
PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
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