Objective To investigate the mucosal immunity of L1 virus-like particles ( VLPs) of human papillomavirus ( HPV) types 16 and 18 plus JY adjuvant by intranasal immunization in cynomolgus. Methods Cynomolgus were immunized with low and high dosage of HPV types 16 and 18 L1 VLP with JY adjuvant for 3 times by intranasal route at weeks 0, 4 and 8, respectively, using PBS as control. Subsequently, vaginal secretion, oral secretion and nasal secretion were collected at weeks 0, 2, 4, 6, 8 and 16, respectively, and determined for mucosal immunity by ELISA.Results HPV-L1-specific sIgA antibodies were detected in all secretions, including oral, nasal and vaginal ones, the concentrations of sIgA antibody induced were much higher than those in PBS control group.There was no significant difference ( P>0.05) in sIgA antibody levels among cynomolgus vaccinated with low and high dosage of L1 VLP, as was between oral and nasal secretion ( P >0.05 ) , However, the concentrations of sIgA antibody in vaginal secretion were significant higher than those in oral and nasal secretion, differences were statistically significant ( P<0.01) .Conclusions Following intranasal immunization in cynomolgus, HPV types 16 and 18 L1 VLP with JY adjuvant can effectively induce sIgA antibody in vaginal secretion, and vaginal sIgA antibody concentrations were much higher than those in oral and nasal secretion.%目的：探讨人乳头瘤病毒16和18型L1病毒样颗粒与JY佐剂联合（ HPV16＋18 L1 VLP＋JY佐剂）鼻腔免疫食蟹猴的黏膜免疫效果。方法分别用低剂量和高剂量 HPV 16＋18 L1 VLP＋JY佐剂疫苗鼻腔喷雾免疫食蟹猴3次（第0、4、8周），并设PBS对照组，于第2、4、6、8、16周采集阴道分泌物、鼻腔分泌物和口腔分泌物，ELISA法检测sIgA抗体浓度。结果低剂量和高剂量HPV 16＋18 L1 VLP＋JY佐剂疫苗均诱导鼻腔、口腔和阴道分泌物产生sIgA抗体，均高于对照组（ P＜0.01）；低剂量组和高剂量组sIgA抗体水平差异无统计学意义（ P＞0.05），阴道分泌物sIgA抗体水平高于鼻腔与口腔分泌物sIgA抗体水平（P＜0.01），鼻腔分泌物sIgA抗体水平与口腔分泌物sIgA抗体水平差异无统计学意义（ P＞0.05）。结论含有JY 佐剂的 HPV 16和18型 L1 VLP 鼻腔喷雾免疫食蟹猴，能够有效诱导阴道黏膜产生 sIgA 抗体，且高于口腔和鼻腔黏膜 sIgA 抗体浓度。
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