Peroxisome Proliferator-Activated Receptor Alpha (Pparα) in Redlip Mullet, Liza Haematocheila：Molecular Cloning, Mrna Tissue Expression, and Response to Dietary Lipid Levels

摘要

The full-length cDNA of proliferator-activated receptor alpha (PPARα) was abtained from the liver of redlip mullet, Liza Haematocheila. The PPARα cDNA (GenBank no: KJ848472) was 2409 bp including a 1437 bp open reading frame, which encoded 478 amino acids with four signature domains, i.e., the hypervariable region in N-terminus, DNA-binding domain (DBD), flexible hinge domain and ligand-binding domain (LBD). The mRNA expression level of PPARα was detected in all tissues tested. Highest expression occurred in liver, followed by brain, stomach, skin, spleen and visceral fat, but the expression was weak in heart and muscle. Then, a 60-day feeding trial was conducted to study, the effects of dietary lipid levels (2.0%, 4.8%, 7.5%, 9.8%, 12.0% and 14.6 %) on the mRNA expression of PPARα in mullet. PPARα mRNA expression in liver increased significantly (P0.05) with the increasing dietary lipid levels. These results indicated that PPARα was tissue-differential expressed gene and played a pivotal role in regulating the lipid metabolism mainly in liver. Results of this study will benefit the further researches on the relationships between PPARα gene and fat metabolism of redlip mullet.