Label-free biochips for rapid detection of soybean allergen GlymBd 30K (P34) in foods

摘要

Purpose: To develop an innovative method for detection of soybean allergen, Gly mBd 30K (P34) in foods using a biosensor based on high spatial imaging ellipsometer. Methods: Two monoclonal antibodies, 2D1 and 5F9, each known to have specific bioactivity against P34 allergen, were selected and separately immobilized as ligands on silicon wafer surface to allow capture of the P34 allergen. The resultant changes on the wafer surface were viewed directly as images in gray scale. Results: Images indicated that these two antibodies detected the presence of P34 allergen in soybean extract with sensitivity of 1 mg/L and a detection time of about 15 min. For the detection of P34 allergen in foods, results from biochip detection were consistent with those obtained using ELISA detection. Conclusion: These results show that the biochip may be an effective analytical tool for food allergen detection.