An improved primary culture method for hippocampal neurons in fetal rats and MAP2 identification

摘要

Abstract Objective: To establish a simple, effective and high-purity primary culture method for fetal rat hippocampal neurons. Methods: Wistar rats of gestational age 18 days were taken and the brain tissue was separated under the microscope. Single neuronal cells were obtained by digestion with Brain Dissociation Kit, and then were seeded in cell plates to observe the basic morphologic structure after 24h, 3d, and 5d. Immunofluorescence of microtubule associated protein 2 was applied to assess cell purity of the culture. Results: The hippocampal neurons obtained in this culture method are in good condition and grow vigorously. On the 7th day after culture, the purity of neurons was up to 99.62%. Conclusion: The method is simple and effective for obtaining the high-purity and stable neurons.