Effects of n-Octyl-β-D-Glucopyranoside on Human and Rat ErythrocyteMembrane Stability Against Hemolysis

摘要

The practical importance for the pharmaceutical and cosmetics industries of the interactions between biologicalmembranes and surfactant molecules has led to intensive research within this area. The interactions of non-ionic surfactantn-octyl-β-D-glucopyranoside (OG) with the human and rat erythrocyte membranes were studied. The in vitro hemolyticand antihemolytic activities were determined by employing a method in which both erythrocytes were added to thehypotonic medium containing OG at different concentrations, and the amount of haemoglobin released was determined. noctyl-β-D-glucopyranoside was found to have a biphasic effect on both types of erythrocyte membrane. We alsoinvestigated the interactions of OG with the erythrocyte membrane in isotonic medium; the dose-dependent curves showsimilar behaviour in both human and rat erythrocytes. Our results showed that OG has greater antihemolytic potency onrat than on human erythrocytes; furthermore, rat erythrocytes were more sensitive than human erythrocytes to hypotonicshock. How the different lipoprotein structure of these erythrocytes determines a difference in antihemolytic activity isdiscussed.