C-Kit Ligand Promotes Mast Cell Infection by Toxoplasma gondii

摘要

Biological functions of mast cells include a functional role in innate immunity against parasitic infections.Here, we demonstrated that mast cells could also play a role in the anti-microbial defenses regulation and might participateas a parasite reservoir. We observed that Toxoplasma gondii infected massively in vitro mouse bone marrow derivedmast cells (BMMC), a mucosal mast cell (MMC) phenotype, followed by substantial cell lysis. This induced release of ??-hexosaminidase, but not of preformed or neosynthesized TNF-. Culturing MMC in the presence of recombinant mousestem cell factor (c-kit ligand) led to their maturation into connective tissue-like mast cells (CTMC), which T. gondii wasable to adhere on and to infect more. T. gondii infection did not induce release of ??-hexosaminidase and serotonin fromBMMC. These results demonstrated that mast cells interact with T. gondii and are massively infected, especially aftertheir maturation by c-kit ligand.