Effect of Different Strength of Medium on Organogenesis, PhenolicAccumulation and Antioxidant Activity of Spearmint (Mentha spicata l.)

摘要

We investigated the effect of inorganic salt concentration on the in vitro organogenesis of spearmint (Menthaspicata L.) by using Murashige and Skoog culture medium of different strength (full, half- and quarter strength). Thehighest number of shoots and roots induced per explant (3.5 and 10, respectively), as well as the maximum average shootlength (16 cm) was observed on half strength medium. The maximum leaf number (35 per explant) and average rootlength (7 cm) were associated with regeneration on full medium. The highest average phenolic content was observed onshoot extracts (7.20 mg/g f.w.) and root extracts (5.93 mg/g f.w.) cultured onto quarter and half strength medium,respectively. The strength of the culture medium was inversely correlated with the antioxidant activity of plant extracts.Therefore, for spearmint organogenesis, half strength MS medium offers a compromise between optimum growth in vitroand antioxidant phenolic accumulation.