DETEKCIA A IDENTIFIKáCIA DRUHU LACTOBACILLUS V GASTROINTESTINALNOM TRAKTE KUR?IAT ZA POU?ITIA METóD FISH A PCRDETECTION AND IDENTIFICATION OF LACTOBACILLUS SPECIES IN GASTROINTESTINAL TRACT OF CHICKEN BY FISH AND PCR ANALYSIS

摘要

The aim of the present study was a rapid detection and identification of Lactobacillus sp. in gastrointestinal tract of chickens by fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) analysis. As a biological material broiler chickens Hybro were used. They were fattening by the combined probiotic preparation (1st and 2nd experimental group) for elimination of pathogens and better utilization of feed. The 100 % of isolated strains covered Lactobacillus salivarius and Lactobacillus acidophilus. For quantification of bacterial count was used FISH method. The statistical evaluation showed significant differences (P≤0.001) between control group and first experimental group in the number of Lactobacillus sp. counts. The same differences were found between control group and second experimental group. In the number of Lactobacillus sp. counts were found statistical significant differences (P≤0.05) between first and second experimental group.