Analysis of GDSL lipase (GLIP) family genes in rice (Oryza sativa)

摘要

Lipase is one of the lipid hydrolyzing enzymes, distributed broadly throughout plants, animal and microorganisms. GDSL-lipases is one of the lipases that exhibit a GDSL motif GxSxxxxG, in which the active site Serine is located near the N-terminus and display a Gly-Asp-Ser-(Leu) [GDS(L)] motif in conserved block I. However, the knowledge about their roles in developmental processes and response to various stimuli are still very limited in rice. A systematic analysis revealed the presence of at least 113 GDSL lipase (GLIP) genes in the rice genome. The tandem gene duplications have contributed a major role in expansion of this gene family. Phylogenetic analysis classed proteins into three groups; OsGLIP group B contained 56 genes, 50 in group A and only 2 genes in group C. The organization of putative motifs indicated potential diverse functions of GLIP gene family members in rice. Microarray data analysis revealed tissue and developmental stage-specific expression patterns of several OsGLIP genes. 38 OsGLIP genes were especially expressed in stigma and seed germination, several genes expressed exclusively in root and 17 OsGLIP genes were induced by any of three stresses. Our analysis also suggests differential accumulation of cluster genes during these processes. Our analyses indicated OsGLIP genes may have potential roles in rice development and abiotic stresses.