Natural Infection of Datura stramonium L. By an Unusual Strain of Pepper veinal mottle virus Genus Potyvirus in Nigeria

摘要

A disease causing mosaic and leaf distortion was observed in 80% of Datura stramonium L. plants in a garden in Ibadan, Nigeria. The causal agent had flexuous rod-shaped particles of about 750-800 nm length. The virus was readily mechanically transmissible but was not seed borne in the natural host. The virus was not transmissible to any other host genera and was restricted to D. stramonium and D. metel . In serological tests using protein A-sandwich enzyme-linked immunosorbent assay (PAS-ELISA) and antisera to ten members of the family Potyviridae , the virus from D. stramonium reacted only with the antiserum to Pepper veinal mottle virus (PVMV) genus Potyvirus . Optical density readings of 3.564 and 0.466 at 405 nm were obtained in reactions between PVMV antiserum and virus infected and healthy Datura sp. samples respectively. No serological relationship was detected between the virus from D. stramonium and Blackeye cowpea mosaic virus (BICMV) genus Potyvirus , Cowpea aphid-borne mosaic virus (CABMV) genus Potyvirus and Yam mosaic virus (YMV) genus Potyvirus in double antibody sandwich ELISA (DAS-ELISA) and immunosorbent electron microscopy (ISEM) tests. Although some of the data on symptomatology, host range and serological relatedness could implicate Datura shoestring virus (DSV) genus Potyvirus , the virus may actually be an unusual strain of PVMV.