Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

摘要

Context: Adlay seed [Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf (Poaceae)] is a Traditional Chinese Medicine, which has been investigated to treat inflammatory diseases and rheumatism.Objective: This study evaluates the ameliorative effects of adlay seed extract (ASE) in a complete Freund's adjuvant (CFA)-induced rheumatoid arthritis (RA) rats.Materials and methods: The RA Sprague-Dawley rat model was induced and randomly divided into six groups with or without ASE treatment (50, 100 or 200?mg/kg). After 28?d administration, the symptoms, biochemical parameters and molecular mechanisms were investigated.Results: The values of paw oedema, PGE2 and MMP-3 decreased from 1.46?±?0.04 to 0.66?±?0.07?cm3, from 126.2?±?11.48 to 79.71?±?6.8?pg/mL and from 142.7?±?8.36 to 86.51?±?5.95?ng/mL, respectively; the values of body weight increased from 177.25?±?5.94 to 205?±?6.52?g in HASE group. In addition, treatment of ASE reduced the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, MCP-1), and increased the activities of antioxidant enzyme (GSH-Px, SOD, and CAT). Furthermore, ASE could suppress the mRNA expression of COX-2 and CHI3L1 and improve the mRNA expression of CAT and GPx-1 in ankle tissues of RA rats.Discussion and conclusions: For the first time, our results indicated ASE exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress. Our finding sheds light on the research and development of anti-RA functional foods from adlay seed.