Development of HPTLC method for determination of cefpodoxime proxetil and ambroxol hydrochloride in human plasma by liquid–liquid extraction

摘要

Aim: To develop a simple, sensitive, rapid, and economic high performance thinlayer chromatographic method for determination of cefpodoxime proxetil (CEFPO)and ambroxol hydrochloride (AMBRO) in human plasma by liquid–liquid extractionusing paracetamol as an internal standard. Materials and Methods: The plasmasample was extracted by a mixture of methanol and acetonitrile. A concentrationrange from 500 to 3500 ng/spot for CEFPO and 1000 to 7000 ng/spot for AMBROwere used for the calibration curve, respectively. This recovery was found to be 74.40and 94.50 for CEFPO and AMBRO, respectively. The mobile phase used consistsof chloroform: methanol (9:1v/v). Densitometric analysis was carried out at awavelength of 240 nm. Results: The Rf values for CEFPO, AMBRO, and paracetamolwere found to be 0.69 ± 0.005, 0.49 ± 0.0057, and 0.31 ± 0.0054, respectively. Thestability of CEFPO and AMBRO in plasma was confirmed during three freeze–thawcycles (-20°C), on bench during 24 h and post preparative during 48 h. Conclusion:The proposed method was validated statistically and by performing a recoverystudy for determination of CEFPO and AMBRO in human plasma.