BIOAVAILABILITAS TABLET IBUPROFEN PADA PEMBERIAN BERSAMAAN DENGAN EKSTRAK AIR HERBA PEGAGAN (Centella asiatica (L) Urban) PADA KELINCI JANTAN

摘要

The tendency to use herbal drug simultaneously with synthetic drug may result in an interaction. Based on research, aqueous extract of Centella asiatica (L) Urban contains saponin (asiaticoside and madecassoside) compounds that inhibit CYP450 and is a safe herbal alternative that is used for anti-inflammatory treatment. The aim of this study was to investigate the effect of aqueous extract of Centella asiatica (L) Urban to the ibuprofen tablet bioavailability in male rabbits. Both compounds are metabolized by CYP450 enzymes in the same way with different mechanism. The cross over design with the same subject was used in this study. The samples consisted of 400 mg ibuprofen with 0%b/v, 25%b/v, 50%b/v dan 100%b/v aqueous extract of Centella asiatica (L) Urban respectively. The blood were taken after 0; 0,5; 1; 1,5; 2; 2,5; 3; 3,5; 4; 6; 8; 10 hours after samples treatment, then the ibuprofen plasma concentration were determined spectrophotometrically. The results then were used to evaluat the bioavailability parameters: tmaks, Cpmaks, and AUC and were analyzed using ANOVA test in the same way as well as the 95% trust standart. The results showed, the use of ibuprofen tablet stimultaneously with aqueous extract of Centella asiatica (L) Urban increased Cpmaks dan AUC, but there were no influence to the tmaks value. The use of ibuprofen tablet stimultaneously with 50 %b/v and 100 %b/v aqueous extract of Centella asiatica (L) Urban had a significant influence to the bioavailability of ibuprofen.