EFEK PROTEKTIF EKSTRAK ETANOL HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PEMBENTUKAN KATARAK TIKUS WISTAR YANG DIINDUKSI SODIUM SELENIT

摘要

Cataract is one of the eye pathology that can cause blindness. Oxidative stress has been identified as an initiating factor of cataract. The objective research was to study the protective effect of ethanol extract Centela asiatica toward cataract formation on Wistar rat induced by sodium selenite. Cataract was induced in 9 days old Wistar rat, which 5 groups of 7 each. Group I got injection aquadest and the test groups got injection CMC-na (group II), vitamin E dose 378 IU/kg bw (group III), ethanol extract Gotu cola herb dose 100 mg/kg bw (group IV), and 200 mg/kg bw (group V), 400 mg/kgbw, subcutaneously, respectively, for 3 days. After 4 hours, all rats got injection a single dose 50 ug/kg of BW sodium selenite intraperitoneally. Then eyes were observed in all groups on postnatal day 16, when the eyes of the rat first opened. Then percentage of cataract incidence were calculated. Finally, rats were executed and eyes were taken for lens histophatological analyses with hematoxiline eosin staining. Based on macroscopic and microscopic analyses it can be concluded that ethano extract of Centella asiatica had protective effect toward cataract formation on Wistar rat induced by sodium selenite. The least cataract incidence was showed at dose 200 mg/kg bw.