Effect of colchicine on in vitro polyploidy induction in African Marigold (Tagetes erecta)

摘要

Colchicine was used as chemical mutagen for In vitro induction of polyploids. Noda l segments of In vitro grown marigold plantlets were cultured on MS medium supplemented with four different concentrations of colchcine (0.0%, 0.001%, 0.01% and 0.05%) for 12h, separately. After treatment, nodal segment were shifted to colchicine free MS medium for normal growth. Highest mortality rate (72.74%) and restricted shoot growth (3.80 cm) was observed in 0.05% colchicine treatment. Stomatal size and number used as cytological parameters to identify polyploids. Stomatal size was increased in 0.01% (811.34 μm) and 864.70μm in 0.05% treatments while 289.30 μm was in c ontrol. The stomatal number was reduced to 9 in 0.05% colchicine treatment as compared to control (16). The polyploids showed an increase in stomatal size and decrease in stomatal number per unit area. Thus 0.01% and 0.05% treatments found effective in inducing polyploidy in African marigold.