Incidence of β-lactamases Among Novel Multidrug Resistant Clinical Isolates of UTI Patients: An Antimicrobial Resistance Surveillance Study

摘要

Community or Nosocomial Urinary Tract Infections (UTI) are increasing worldwide speedily by creating problem to currently available β-lactam antibiotic therapies. Thus it is an urgent need of research to find the effective therapeutic options to treat / manage the UTI. Therefore, the present work is designed to determine prevalence of β-lactam resistance in seven novel bacterial isolates from the community UTI as well as to identify and characterize the types of β-lactamases involved in UTI. Total Five Hundred and Forty Nine (n= 549) clinical strains isolated from Urine samples and identified as E. coli, P. mirabilis, K. pneumoniae, P. aeruginosa, Enterobacter spp., Escherichia spp., Enterococcus spp., A. haemolyticus, Micrococcus, Serratia spp., Shigella spp., Staph. Aureus etc. Antimicrobial susceptibility profile of these strains revealed high level resistance to Ampicillin (99%), Ceftazidime (98.99%), Amoxicillin (98%), Cefotaxime (99%), Cefaclor (98%) and Penicillin (99%). Thus out of 549 only seven were detected as novel Extended Spectrum β-lactamase producers. All the seven isolates were identified at 16 S r RNA level and submitted to NCBI with following accession numbers such as: JX827388.1, JX827385, JX827384.1, JX827383.1, JX827382, JX827386 and JX827387. Minimum inhibitory concentration of each novel strain was determined against 25 different β-lactam antibiotics i.e. Ampicillin, Amoxiclav, Oxacillin, Vancomycin, Gentamycin, Ciprofloxacin, Nitrofurantoin, Ceftazidime, Cotrimaxazole, Trimethoprim, Imipenam, Amikacin, Amoxicillin, Cephalexin, Cefuroxime, Nalidixic acid, Cefazoline, Cefdinir, Cefexime, Ceftriaxone, Cefuroxime, Cefotaxime, Aztreonam, Cefaclor, Penicillin etc. All these strains revealed to possess virulence markers such as α-hemolysin, Cell surface hydrophobicity, Aerobactin production, Serum Resisitance, Colicine production etc. In addition to this an antimicrobial surveillance of the same strain was tested for multiple antibiotic resistance scale with the help of WHONET software designed by World Health Organization.