Okabe and Tanaka (1958) reported the urine precipitin reaction for schistosomiasis japonica in 1958. In the field studies the authors sometimes met negative reaction among school children. A teacher asked children to bring the first morning urine for test. When children forgot the first urine, they might have brought the second or third urine specimen to our experiment. Experiments were conducted to examine the entire day's urine of schistosomiasis patients. At first sodium azide was used for the preservation of urine. This chemical is recently difficult to get, so merthiolate (Japanese name, Merzonin) was employed. Immunization of rabbits with the schistosomiasis patient's urine was tried in order to prove the presence of the antigen in the urine and to obtain antiserum for Schistosoma japonicum antigen.
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机译：Okabe and Tanaka（1958）在1958年报道了日本血吸虫病的尿沉淀反应。在田野研究中，作者有时遇到在学童中的阴性反应。一位老师要求孩子们在第一天早上带尿进行测试。当孩子忘记第一尿液时，他们可能已经将第二或第三尿液标本带到了我们的实验中。进行了实验以检查血吸虫病患者全天的尿液。首先，叠氮化钠用于尿液的保存。这种化学药品最近很难获得，因此使用了硫柳汞（日本名Merzonin）。尝试用血吸虫病患者的尿液免疫兔，以证明尿液中存在抗原，并获得针对日本血吸虫抗原的抗血清。
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