Experimental research for determination of bisoprolol fumarate in human plasma samples using liquid chromatography- tandem mass spectrometry (LC-MS/MS) technique

摘要

Liquid chromatography coupled with mass spectrometry detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, rapid method development. In this context, a liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of bisoprolol in human plasma samples, using metoprolol as internal standard. The assay has proven to be sensitive, specific and reproducible, suitable to determine the bisoprolol concentration, following a single oral administration of a 10 mg bisoprolol tablet in 22 healthy volunteers,.in the bioequivalence study of Bisoprolol 10 mg coated tablets, produced by Antibiotice S.A. versus Concor? 10 mg, produced by Merck.