Comparative in vitro study of the chitosan application method effect on Aspergillus brasiliensis growth

摘要

The aim of this study was to investigate the in vitro antifungal activity of medium molecular weight chitosan and chitosan film obtained by solvent casting method against Aspergillus brasiliensis ATCC 16404. Five methods were used: incorporation of Aspergillus brasiliensis in Potato dextrose agar (PDA) and puncture a solution of chitosan in acetic acid 1% in the centre of Petri dish(1), spreading the spore suspension on the entire agar surface of a Petri dish and placement of the chitosan solution in the centre of the plate (2), inoculation of the spore suspension in the centre of the Petri dish and applying 6 mm diameter disks with chitosan solution onto the agar surface (3), spreading the spore suspension on agar media followed by making bores which were loaded with chitosan solutions (4), incorporation of chitosan solution in PDA media followed by puncture inoculation with spores in the centre of the Petri dish (5). In case of the chitosan films, the method used consist in the inoculation of media with spore suspension in the centre of the dish after which the films were applied on the surface, in contact with the spore inoculum. Plates were incubated at 25°C and the growth was monitored for 7 days.