Hormone receptor type 2 antimüllerian gene role in dogs with Persistent Müllerian Ducts Syndrome

摘要

Persistent Müllerian Ducts Syndrome (PMDS) is a congenital disease, characterized by thepresence of Müllerian duct derivatives in genetically defined males. Our aim was to identify DNAmutations that may lead to this disease in Basset Hound male dogs. Six males (one healthy control)aged 1–8 years were examined by ultrasound; exploratory laparotomy was used for PMDSconfirmation in dogs detected as PMDS positive following ultrasound examination. Surgical treatmentwas performed for resection of uterine horns rudiments. Two dogs were found to be PMDS-positive.Possible DNA mutations in the 11 exons of the hormone receptor type 2 antimüllerian (AMHR2) genewere analyzed by polymerase chain reaction (PCR) followed by DNA sequencing. All exons of AMHR2gene appeared wild-type, unmodified, suggesting that AMHR2 gene mutations may not be present inBasses Hound male dogs with PMDS. These findings suggest that another gene mutation (e.g. AMH)may be involved in PMDS occurrence in Basset Hound breed and we are currently investigating thisalternative direction.