This cross-sectional study aimed to identify the prevalence of smoking among employees of a university hospital in Southern Brazil. Data collection happened in 2008, during the periodic health exam, using a questionnaire, according to the smoking status of the employees. The sample consisted of 1,475 subjects, in which 979 (66.4%) were non-smokers, 295 (20%) former smokers and 201 (13.6%) smokers. Smoking was more prevalent among employees with lower education levels and among professionals in administrative positions. Among smokers, low dependence was identified, as well as desire and high degree of motivation to stop smoking, with health concerns as the main reason. Thus, taking into account the motivation of smokers to stop smoking, this is an appropriate time for health education and specific support to employees in the process of smoking cessation.
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