Disgregado para microscopía de tejidos le?osos utilizando monoetanolamina en medio alcalino

摘要

This work is based on the need to have a method for the isolation of lignocellulosic fibrous material formicroscopy, without damaging the cells so much, which can be carried out in a short time in the laboratory, atatmospheric pressure, and without toxic. A procedure which consists of treating wood with sodium hydroxideinto monoethanolamine at 170o C for 1 to 4 hours was developed. A variety of woods could be isolated- softwoodsas well as hardwoods. The amount of alkali used was 25% on dry wood, with a liquor-wood relationship of 10. Aquantitative analysis of fibers gave as a result between 4 and 5% of broken elements. A direct relationship betweenwood density and the time required to complete the treatment was found, being of 1 hour for the 0.14 Kg/dm3density, and of 4 hours for the 0,80 Kg/dm3 density.