NADPH oxidase-derived H₂O₂ mediates the regulatory effects of microglia on astrogliosis in experimental models of Parkinson's disease

摘要

Highlights ? Microglia are capable of regulating the immunological functions of astrogliosis in Parkinson's disease. ? NADPH oxidase-derived H₂O₂ is recognized as a paracrine signal for microglial regulation of astrogliosis. ? Transcription factors STAT1 and STAT3 play pivotal roles in H₂O₂-induced astroglial activation. Astrogliosis has long been recognized in Parkinson's disease (PD), the most common neurodegenerative movement disorder. However, the mechanisms of how astroglia become activated remain unclear. Reciprocal interactions between microglia and astroglia play a pivotal role in regulating the activities of astroglia. The purpose of this study is to investigate the mechanism by which microglia regulate astrogliosis by using lipopolysaccharide (LPS) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse PD models. We found that the activation of microglia preceded astroglia in the substantia nigra of mice treated with either LPS or MPTP. Furthermore, suppression of microglial activation by pharmacological inhibition or genetic deletion of NADPH oxidase (NOX2) in mice attenuated astrogliosis. The important role of NOX2 in microglial regulation of astrogliosis was further mirrored in a mixed-glia culture system. Mechanistically, H₂O₂, a product of microglial NOX2 activation, serves as a direct signal to regulate astrogliosis. Astrogliosis was induced by H₂O₂ through a process in which extracellularly generated H₂O₂ diffused into the cytoplasm and subsequently stimulated activation of transcription factors, STAT1 and STAT3. STAT1/3 activation regulated the immunological functions of H₂O₂-induced astrogliosis since AG490, an inhibitor of STAT1/3, attenuated the gene expressions of both proinflammatory and neurotrophic factors in H₂O₂-treated astrocyte. Our findings indicate that microglial NOX2-generated H₂O₂ is able to regulate the immunological functions of astroglia via a STAT1/3-dependent manner, providing additional evidence for the immune pathogenesis and therapeutic studies of PD.