Genetic polymorphisms of growth hormone (GH) gene in Kacang goat population based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) identification

摘要

KacanggoatisasmallruminantnativefromIndonesiaandspreadwidelyinseveralSoutheastAsiancountries,andhasbecomepartoftheworld#8217;sbiodiversity.GrowthHormone(GH)ingoatisaproteinsecretedfromanteriorhipophysebysomatrotopcellsanditsformationprocessunderthecontrolofGHgene.Itsmainfunctionistostimulatethegrowthofbone,muscleandfatmetabolism.ThepurposeofthisstudyistoidentifytheGHgenepolymorphisminKacanggoatwithPolymeraseChainReaction-RestrictionFragmentLengthPolymorphism(PCR-RFLP)methods.Atotalof168bloodsamplesfromKacanggoatintworegionsofIndonesia(GorontaloandSouthSulawesiprovince)wereextractedbyusingDNAextractionkittocollectwholeDNAgenome.AmplificationandgenotypingofGHgenewasperformedusingPCR-RFLPusingtheHaeIIIrestrictionenzyme.Theresultsobtainedtwokindsofgenotypes,withtheAAgenotypefrequencies(0.095)andAB(0.904).ThefrequencyofalleleA(0.547)andB(0.452)indicateapolymorphismintheGHA781GlocusinKacanggoat.Theobserved(Ho)andexpected(He)heterozygosityvaluewererespectively0.0904and0.496.GHallelesdistributioninKacanggoatpopulationswerenotinHardy-Weinbergequilibrium.Basedonstatisticalanalysis,thereisnodifferencebetweenbodysizeofgenotypeAAandAB.TheresultsofthisstudycanbeusedasinitialinformationinformulatingastrategyforKacanggoatbreedingbyutilizingMarkerAssistedSelection(MAS).