METHOD FOR STRAIN DISTINCTION OF CHRYSANTHEMUM CHLOROTIC MOTTLE VIROIDS USING POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM

摘要

The present invention relates to a method for discriminating a mutant strain of Chrysanthemum chlorotic mottle viroid (CChMVd) using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The method according to the present invention is used to effectively discriminate msim34, SSHA6, Beijing, India 669541, and India 646404, which are five mutant strains of CChMVd. Therefore, Chrysanthemum infectious viroids that seriously damage Chrysanthemum farmhouses can be detected early, promptly, and accurately, thereby expected to significantly reduce a huge economic loss.