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Kanniadu goats of Tamilnadu, India: genetic characterisation through microsatellite markers

机译:印度泰米尔纳德邦的肯尼亚杜山羊:通过微卫星标记的遗传表征

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摘要

CharacterisationofKanniadugoatswasdoneusingmicrosatellitemarkers.ThegenomicDNAfrom50unrelatedKanniadugoatswerePCR-amplifiedwithapanelof20microsatellitemarkersandresolvedthrough6percentdenaturingpolyacrylamidegelelectrophoresisfollowedbysilverstaining.Thenumberofallelesrangedfrom5to14withallelesizesrangingfrom90to222bp.Theallelefrequenciesrangedfrom0.0106to0.4480.Polymorphisminformationcontentrangedfrom0.5710to0.8570.Exceptfourloci,thepopulationwasnotinHardy-Weinbergequilibrium.Theobservedheterozygosityrangedfrom0.7142to0.9778whiletheexpectedheterozygosityrangedfrom0.6390to0.8702,indicatingtheheterogenousnatureofthepopulationdistributedinthebreedingtract.
机译:CharacterisationofKanniadugoatswasdoneusingmicrosatellitemarkers.ThegenomicDNAfrom50unrelatedKanniadugoatswerePCR-amplifiedwithapanelof20microsatellitemarkersandresolvedthrough6percentdenaturingpolyacrylamidegelelectrophoresisfollowedbysilverstaining.Thenumberofallelesrangedfrom5to14withallelesizesrangingfrom90to222bp.Theallelefrequenciesrangedfrom0.0106to0.4480.Polymorphisminformationcontentrangedfrom0.5710to0.8570.Exceptfourloci,thepopulationwasnotinHardy-Weinbergequilibrium.Theobservedheterozygosityrangedfrom0.7142to0.9778whiletheexpectedheterozygosityrangedfrom0.6390to0.8702,indicatingtheheterogenousnatureofthepopulationdistributedinthebreedingtract。

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