Methods to improve the in vitro culture of GF677 (peach × almond) peach rootstock

摘要

Abstract In this study, the effect of exogenous application of gibberellic acid (GA3) and the presence of both leaves and petioles on sprouting of the rosetting plantlets of GF677 (peach × almond hybrid) was examined. GA3 caused a significant increase in sprouting of rosetting plantlets at application rates of 12.5 and 50 mg litre–1. The results showed that rosetting plantlets without leaves and petioles, or with only one petiole, sprouted significantly better compared with those with one‐quarter of leaf, all petioles, or all petioles and leaves. In addition, Mg + Zn + B (trace) in Murashige & Skoog medium significantly increased the fresh weight and water content of plantlets.