Purpose: A lot of accidents occur in the laboratory due to lack of proper knowledge regarding laboratory safety measures, indifferent attitude & improper implementation of safe laboratory practices. In view of this, the present study on knowledge, Attitude & Practice (KAP) of laboratory safety measures was carried out among paramedical staff of laboratory services of tertiary care teaching hospital, western India. Method: This was a comparative study which used a standardized, structured self-administered questionnaire to survey knowledge, attitude and practice of paramedical staff. The KAP study enrolled 81 respondents. Results: Regarding knowledge- the majority knew the very important issues related with laboratory safety like Post Exposure Prophylaxis (96.55%) & discarding of blood samples (93.10%) etc. In regard to attitude towards the scientific process, all are very much aware about importance of protective devices (i.e. Wearing Gloves) and Biomedical waste management. In regard to the practice in laboratory, the entire study subject group (100%) replied “YES” in each question that shows the good quality work of the laboratory. Conclusion: The induction training on Laboratory safety is very important and motivating exercise for improving the laboratory safety measures.
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