Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro

摘要

In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells. Research Highlights During the process of neural stem/progenitor cell differentiation, fibroblast growth factor or Sonic Hedgehog proteins or factors may be secreted from other neural cells, and together they compose a niche environment for neurosphere co-culture.The experimental results were analyzed by reverse transcription-PCR, western blot and enzyme-linked immunosorbent assay, to provide a detailed analysis of mRNA and protein expression. There were differences between the cell mRNA, protein content and protein secretion levels. Abbreviations ELISA, enzyme-linked immunosorbent assay