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首页> 外文期刊>Mitochondrial DNA Part B >Characterization of the complete chloroplast genome of longan (Dimocarpus longan Lour.) using illumina paired-end sequencing
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Characterization of the complete chloroplast genome of longan (Dimocarpus longan Lour.) using illumina paired-end sequencing

机译:利用照明双末端测序技术表征龙眼的完整叶绿体基因组

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AbstractLongan (Dimocarpus longan Lour.), as a kind of commercial fruit tree in the family Sapindaceae, is widely cultivated in South Asia. In this study, we obtained the complete chloroplast genome sequence of longan using Illumina paired-end sequencing. It has 160,833?bp in length, containing a pair of IR regions (28,428?bp) separated by a small single-copy region (18,270?bp) and a large single-copy region (85,707?bp). The overall GC contents of the chloroplast genome were 37.8%. This circular genome contains 130 annotated genes, including 85 protein-coding genes, 37 tRNAs and 8 rRNAs. The phylogenetic analysis using maximum–likelihood (ML) and neighbour-joining (NJ) method showed that longan has the closest relationship with Litchi chinensis, Sapindus mukorossi and Dodonaea viscosa. This complete chloroplast genomes can be subsequently used for the genetic breeding of this valuable species.
机译:摘要龙眼(Dimocarpus longan Lour。),作为无患子科的一种商业果树,在南亚广泛种植。在这项研究中,我们使用Illumina的双末端测序获得了龙眼的完整叶绿体基因组序列。它的长度为160833 bp,包含一对IR区(28,428 bp),该IR区被一个小的单拷贝区域(18,270 bp)和一个大的单拷贝区域(85,707 bp)隔开。叶绿体基因组的总GC含量为37.8%。该环状基因组包含130个带注释的基因,包括85个蛋白质编码基因,37个tRNA和8个rRNA。用最大似然法和邻域邻接法(NJ)进行系统发育分析表明,龙眼与荔枝,无患子和粘胶十二指肠关系最密切。此完整的叶绿体基因组可随后用于该有价值物种的遗传育种。

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