Restriction fragment length polymorphisms in the apolipoprotein B gene in survivors of myocardial infarction

摘要

Introduction: The aim of this study was to investigate the association between Pvu II and Msp I polymorphisms of the human apolipoprotein B gene and risk of myocardial infarction in 90 survivors of myocardial infarction. Apolipoprotein B is important in the metabolism of lipoproteins and there is an evidence suggesting that this apolipoprotein plays a central role in atherogenesis. Some polymorphisms in the apolipoprotein B gene are associated with peripheral arterial disease, coronary artery disease and risk of myocardial infarction.Material and methods: DNA was prepared from the whole blood. Samples from patients and control group were digested with Pvu II restriction enzyme. Filters were prepared by Southern blotting technique and hybridized with ApoB probe (LB25-A). Genotypes for Msp I polymorphism were determined with polymerase chain reaction.Results: The frequency of the rarer allele (P2) for Pvu II polymorphism in the apolipoprotein B gene was significantly higher in myocardial infarction group (P = 0.001) compared with healthy individuals. A significant association was also found between P2 allele and the age at which myocardial infarction occurred.Conclusion: The results suggest that in Polish population the individuals with P2 allele of the apolipoprotein B gene are at increased risk of developing myocardial infarction. No significant correlation with myocardial infarction event was found for the Msp I polymorphism.