Study on th method performances for screening and confirmatory analysis of aflatoxin M1 in raw milk according to the mycotoxin regulative requirements

摘要

Screning and confirmatory methods for Aflatoxin M 1 (AFM 1 ) analysis were optimized and studied regarding the European Decision 2002/657/EC requirements. The recoveries of AFM 1 from the spiked samples at levels between 0.025 and 0.075 μg kg -1 were 99.6-110.3 % with obtained precision 10.5-13.4 %, confirming the immunochemical method precision and reliability for intensive surveillance studies. According to the regulatory requirements, for confirmation of the obtained positives from the screening, the reference high-performance liquid chromatographic method was evaluated in reference to the performance criteria. The obtained between-day recovery was in the range 67.2-72.8 %, and the precision range 5.4-9.1 %. 205 positives from the screening were subjected to confirmatory analysis. The comparison of the data obtained from the immunoassay and chromatographic method ( R 2 =0.764) revealed a slight overestimation of the screening method, but no cases of false-negative determinations occurred.