BcCFEM1, a CFEM Domain-Containing Protein with Putative GPI-Anchored Site, Is Involved in Pathogenicity, Conidial Production, and Stress Tolerance in Botrytis cinerea

摘要

FIGURE 1. Sequence similarities between BcCFEM1 and other CFEM proteins. (A) Schematic illustrations of BcCFEM1. The domain of BcCFEM1 was predicted by SMART web site. (B) Phylogenetic analysis of BcCFEM1 and CFEM proteins from other fungi. The full length protein sequences were analyzed by MEGA 5 with Unrooted neighbor-joining bootstrap (1,000 replicates). The black triangle indicates BcCFEM1. A scale bar at the lower left corresponds to a genetic distance of 0.1. (C) Multiple sequence alignment of BcCFEM1 and other CFEM proteins. Full-length of protein sequences were aligned using Clustal W and the alignment was edited using Jalview. Eight conserved cysteine residues are highlighted with red triangles. Blue shading intensity reflects level of amino acid identity at each position. Botrytis: BcCFEM1 (BC1G_15201); Sclerotinia: SS1G_07295; Metarhizium: MAM_04868; Phialocephala: PAC_04512; Colletotrichum: CGLO_03584; Trichoderma: TGAM01_02421.