Detection of Enteroviruses in Raw Milk by Nested RT-PCR

摘要

This study aimed to evaluate the incidence of enteroviruses (EV) in milk samples. Raw milk samples(n=150) were collected from different dairy farms, bulk tanks and farmer house (50 sample each) in Kafr-El-Sheikhand Gharbia governorates, Egyptthroughout one year and were analyzed for EVby Nested RT-PCR.Viral RNAwas extracted using BIOZOL total RNA extraction method. The primers were selected from the 5' non-translatedregion, which is the most conserved region in an enterovirus genome. Eleven raw milksamples (7.33%); 4 dairyfarm milk samples (8%), 2 bulk tank milk samples (4%) and 5 farmer house milk samples (10%) were positive forEV RNA.EV were found to be more prevalent in milk fat (4.66%) than the partially skimmed milk (2.66%).This is the first report on the detection of EV RNA incow’sand buffalo’smilk samples in Egypt. This studyhighlights the variations in the incidence of EV in dairy farms, bulk tanks and farmer house raw milksamplesaccording tothe various sources of contamination,the public health significance of EV as well assuggested control and prevention measure to safeguard the consumer were discussed.