Comparative Evaluation of Indirect ELISA, CF Test and PCR for Diagnosis of Ovine Enzootic Abortion ( Ovine chlamydophilosis )

摘要

Chlamydophila (c) abortus is the most important causative agent of the ovine chlamydophilosis,Complement fixation (CF) test is a good screening serological test, but it can be complicated by falsepositive reactions with others species of chlamydophila as C. pecorum and with some Gram negativebacteria. Diagnosis can be improved by using of indirect (I) ELISA. Moderate agreement (kappa-0.6)between CF test and iELTSA was recommended in this study. Blood serum samples from 50 ewes withreproductive disorders and 50 apparently healthy pregnant ewes were investigated by CF test andiELISA to detectct IgG against C. abortus. Sensitivity and specificity of iELISA (90% and 96% respectively)used in this study was found to be higher than CF test (70% and 88% respectively). To confirm theserological results and to evaluate iELISA, five tissue of foeti from aborted ewes with (+ve) CFT and (+ve)iELISA, also five tissue samples of foeti from aborted ewes with negative (-ve) CFT and (+ve) iELISA weresubjected to polymerase chain reaction (PCR). All samples revealed positive results for C. abortus at 119 bp.Therefore, PCR is proved to be specific and accurate technique in the diagnosis of ovine enzootic Chlamydialabortion.