A PCR KIT FOR THE DETECTION OF BOVINE AND OVINE/CAPRINE DERIVED INGREDIENTS

摘要

The present invention provides a polymerase chain reaction (PCR) kit to rapidly detect bovineand ovine/caprine-derived ingredients in one reaction. The PCR kit includes one pair of specific primers, reaction reagents and blank control, as well as positive controls of bovine and ovine/caprine. The “bovine” mentioned in the present invention includes cattle, zebu, yak and buffalo, and the “ovine/caprine” refers to various breeds of sheep and goat. The specific primers are a pair of primers that can amplify the DNA sequence of bovine (SEQ ID NO. 1) and ovine/caprine (SEQ ID NO.2) at the same time. In addition, the size of amplified fragment in bovine (125 bp) differs from that in ovine/caprine (107 bp). Thus, the bovine-derived ingredients could be effectively distinguished from ovine/caprine by detecting the size of amplified DNA fragments in one PCR reaction. The PCR kit can be applied to analyze and determine whether the samples contain bovine or ovine/caprine-derived ingredients. In conclusion, the present invention provides a rapid, sensitive, cost-effective reliable approach for the detection of bovine- and ovine/caprine-derived ingredients.