PCR detection of DNA of bovine, ovine-caprine and porcine origin in feed as part of a bovine spongiform encephalopathy control program.

摘要

The rapid identification of residues of mammalian materials in animal feeds is important for the effective control of feeds as potential sources of transmission of BSE. In this study, a PCR method was tested for the detection of residues from cattle, goats, sheep and swine. DNA extraction was achieved using guanidium thiocyanate, and primers flanking a conserved region of mitochondrial DNA were synthesized for each species. PCR tests were specific for each species and allowed detection in feeds at levels as low as 1% of bovine DNA and 1.5% of ovine, caprine and porcine DNA. It is suggested that these PCR tests may allow the rapid detection of residues of the above species constituting a powerful tool against BSE.