Transcriptome profiling of TDC cluster deletion mutant of Enterococcus faecalis V583

摘要

Abstract The species Enterococcus faecalis is able to catabolise the amino acid tyrosine into the biogenic amine tyramine by the tyrosine decarboxilase (TDC) pathway Ladero et al. (2012) [1]. The {TDC} cluster comprises four genes: tyrS, an aminoacyl-tRNA synthetase-like gene; tdcA, which encodes the tyrosine decarboxylase; tyrP, a tyrosine/tyramine exchanger gene and nhaC-2, which encodes an Na+/H+ antiporter and whose role in the tyramine biosynthesis remains unknown [2]. In E. faecalis {V583} the last three genes are co-transcribed as a single polycistronic mRNA forming the catabolic operon, while tyrS is transcribed independently of the catabolic genes as a monocistronic mRNA [2]. The catabolic operon is transcriptionally induced by tyrosine and acidic pH. On the opposite, the tyrS expression is repressed by tyrosine concentrations [2]. In this work we report the transcriptional profiling of the {TDC} cluster deletion mutant (E. faecalis {V583} ΔTDC) [2] compared to the wild-type strain, both grown in {M17} medium supplemented with tyrosine. The transcriptional profile data of {TDC} cluster-regulated genes were deposited in the Gene Expression Omnibus (GEO) database under accession no. GSE77864.