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Transcriptome profiling for discovery of genes involved in shoot apical meristem and flower development

机译:转录组谱分析,用于发现与茎尖分生组织和花发育有关的基因

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摘要

Abstract Flower development is one of the major developmental processes that governs seed setting in angiosperms. However, little is known about the molecular mechanisms underlying flower development in legumes. Employing RNA-seq for various stages of flower development and few vegetative tissues in chickpea, we identified differentially expressed genes in flower tissues/stages in comparison to vegetative tissues, which are related to various biological processes and molecular functions during flower development. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE42679) and analysis pipeline published by Singh and colleagues in the Plant Biotechnology Journal (Singh et al., 2013), along with additional analysis for discovery of genes involved in shoot apical meristem (SAM) development. Our data provide a resource for exploring the complex molecular mechanisms underlying {SAM} and flower development and identification of gene targets for functional and applied genomics in legumes.
机译:摘要花的发育是控制被子植物种子结实的主要发育过程之一。然而,关于豆科植物花卉发育的分子机制知之甚少。利用RNA-seq进行鹰嘴豆花卉发育的各个阶段和很少的营养组织,我们发现与营养组织相比,花卉组织/阶段的差异表达基因与花卉发育过程中的各种生物学过程和分子功能有关。在这里,我们提供了实验方法,RNA-seq数据(可在GSE42679下的Gene Expression Omnibus数据库中获得)和Singh及其同事在《植物生物技术杂志》(Singh等人,2013)中发布的分析管线的详细信息,以及针对发现与茎尖分生组织(SAM)发育有关的基因。我们的数据为探索{SAM}和花朵发育的复杂分子机制以及鉴定豆类功能基因组和应用基因组的基因靶标提供了资源。

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